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KMID : 0525720170220020118
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Journal of Chitin and Chitosan
2017 Volume.22 No. 2 p.118 ~ p.124
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Production and Properties of Chitinase from Serratia sp. Isolate
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Yoon Ki-Hong
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Abstract
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A bacterial strain capable of hydrolyzing colloidal chitin was isolated from rhizosphere soil of wild medicinal herbs in mountain of Kangwon-do province in Korea by enrichment culture in a minimal medium supplemented with colloidal chitin as a sole carbon source. The isolate, named YB-92, showed similarity higher than 99% with Serratia marcescens and S. nematodiphila on the basis of 16S rRNA gene sequences, assuming the YB-92 belongs to genus Serratia. Chitinase productivity was increased effectively by glucosamine, wheat bran, sucrose and maltose used as additional carbon sources, while the chitinase production was suppressed perfectly by glucose. Particularly, it is worthy of emphasizing that chitinase productivity was not induced by colloidal chitin. Tryptone was suitable nitrogen source for the chitinase production from Serratia sp. YB-92. Maximum chitinase activity was detected in the culture filtrate of media containing tryptone (1.5%) and soytone (0.5%) as nitrogen source. Chitinase production was begun at late log phase of YB-92 with maximal productivity of 14.4 U/mL. The culture filtrate exhibited maximum chitinase activity at 50 ℃ and pH 6.0. The chitinase was stable up to 40 ℃ and retained about 90% of maximal activity after pre-incubation at 45 ℃ for 1 h. The predominant products resulting from the chitinase hydrolysis were oligosaccharides larger than N-acetyl-D-glucosamine for colloidal chitin.
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KEYWORD
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Serratia sp., Chitinase, Characterization, Productivity, Property
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